0049672:Molecular plant Breeding
003579:Molecular Biology in Crop Improvement

Lecture

First Semester, 2007 14.00 – 15.30 Tuesday - friday

Introduction

Gene, Genome, and Plant Breeding (Dr. Apichart Vanavichit)

Lab: Set up molecular mapping experiments

Review basic molecular techniques, Genomic DNA isolation - Genomic DNA isolation

- Genomic DNA isolation

- Gel electrophoresis

Molecular Tools: Molecular Marker Technology

Molecular markers and DNA fingerprinting


- RFLP-based marker

- PCR-based markers

RAPD

AFLP

STS

EST

SSR

SSCP

Lab: Demo (Staffs)

Gene identification known only by phenotype

 

Lab: map construction and QTL analysis (Mr. Meechai)

Lab: Paper discussion (Mr. Meechai, Ms. Nathinee, Ms. Jona)

………………………………..Mid-term Exam……………………………………….

 

 

Lab: Scoring markers and analysis (Mr. Ekawat)

Lab: Pulse field gel electrophoresis, STC marker (Mr. Wintai)

Gene Hunting: Positional cloning (Dr.Apichart Vanavichit)

Lab: Demo High resolution map and discussion

Lab: Demo sequencing Functional genomics (Gene machine)

Lab: Demo SNP, Transposon tagging )

Lab: Demo: Microarray, cDNA selection, SAGE, Differential display

Transgenic Tools : Genetic Engineering (Apichart)

Presentation:

Lab:

…………………………………..Final Exam………………………………….

 

Exam Download

 

 

Grading system

Mid-term exam : 20%

Report : 60%

Final exam : 20%