Phase I - Long-term practical training on Molecular Breeding in Rice for Mekong Region PDF Print E-mail
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Tuesday, 25 May 2010 04:37

Regional Workshops on Molecular Breeding in Rice for Mekong region: Line Conversion Using Marker Aided Selection


SUMMARY


This workshop aims to develop human resources in order to implement marker aided selection (MAS) into the current rice breeding program in the Mekong region, particularly Thailand, Laos, Cambodia, and Myanmar.  Rice breeders have limited knowledge on the use of DNA technology especially molecular marker assisted selection in breeding program.  Although many short-term training on MAS were initiated by international institutions in the past 10 years, few participants especially from developing countries were able to gain enough knowledge or fully understand the practical uses of the technology.  The technology is not implemented in their countries as part of their breeding program.  Not only the limitation of understanding technology but also lack of financial support due to poverty in developing countries thus breeding programs depends on classical plant breeding which takes time to achieve the goals or sometimes with no success.  Currently, Rice Gene Discovery Unit (RGDU) in Thailand had successfully developed and implemented marker aided selection (MAS) in rice breeding program.  These technologies will be useful tools for rice breeders in Mekong region. To successfully transfer MAS technology to breeders in the region, therefore, practical training on MAS is necessary. Breeders attending the workshop will develop useful genetic materials by means of MAS.  We expect that it will be a first-key step to implement MAS into routine breeding program in the region with the hope that it will contribute greatly to the improvement of rice cultivars which directly contribute to the welfare of the farmers through increased rice production and cash income and contribute to the economic development of the region.

 

The expected outputs of the workshop are:

a)      Transfer DNA technology and genomic information from RGDU laboratories into the breeding stations in the region.   This output will be met through practical training by incorporation of biotechnology into conventional plant breeding programs.

b)      Improvement of traditional and/or commercial rice cultivars by using marker assisted selection (MAS).

c)      Enhanced research collaboration between/among institutes within/between countries in the Mekong region.


Introduction

The main objective of implementing MAS in breeding programs is to increase the chance in accumulating favorable alleles of genes beneficial to rice production.  MAS involves 5 step namely:

(1)    Evaluation of genetic resources in order to identify gene/genes, which are important for rice production.

(2)    Establishment of the genetic variation and genetic transfer by inter-crossing between/among rice cultivars.

(3)    Selection of the best progenies containing the target gene/genes from the segregating populations.

(4)    Derivation of the homozygous lines and evaluation of those lines for adaptation, field performance and cooking quality.

(5)    Evaluation of new cultivars for the economic potential at the target environments.

The accuracy and the precision for cultivar selection are keys to the success of the breeding programs.  In the past, breeder selects plants based on its performance (phenotype). This requires knowledge and experience in many areas of plant sciences such as genetic, pathology, entomology, physiology, agronomy, seed technology etc and also knowledge in computational statistic.  A limitation of this method is that efficiency is low in selecting complex phenotypes and it takes a long time to complete the selection.  The limitation is also contributed by G x E interaction and gene action and interaction.  In addition phenotypic selection can not be effectively conducted for a selection of several traits in one time.  Recently, the advance in biotechnology especially in genome and DNA technology can overcome these limitations.  Currently, molecular breeding has been utilized worldwide for plant and animal improvement.  DNA technology together with classical breeding will increase the effectiveness and precision of selection process. Moreover, it will shorten the breeding program and also reduce the cost.

Nowadays, biotechnology plays an importance role in improving food production worldwide.  In Thailand, DNA technology has been utilized for agricultural research in the past 10 years. RGDU is one of the laboratories in DNA research in Thailand specializing in areas of genomics and MAS.  Rice breeding program at RGDU includes the studies on genetic control of many important traits using quantitative trait loci (QTL) approach. QTL information has been utilized for cultivar improvement and MAS has been used to improve traditional cultivars ‘KDML105 and RD6’ for biotic and abiotic resistance. Results were outstanding by increasing the precision and effectiveness of the selection. RGDU aims to transfer the technology to the breeders and researchers who are working in cultivar improvement or related discipline in this regional workshop.  The objective of this regional workshop is to utilize the MAS technology to improve rice cultivars in Mekong region.

Organizing Institute

Rice Gene Discovery Unit (RGDU), National Center for genetic Engineering and Biotechnology, Kasetsart University, Khampangsaen Campus, Nakhon Prathom, Thailand

Funding Agency

-         Rockefeller Foundation

-         Kasetsart University

-         National Center for genetic Engineering and Biotechnology

Lecturers

Name

Institutes

Prof. Dr. Peerasak Srinivej

Kasetsart University

Asst. Prof. Dr. Apichart Vanavichit

Rice Gene Discovery Unit

Dr. Somvong Tragoonrung

DNA Technology Laboratory

Dr. Theerayut Toojinda

Rice Gene Discovery Unit

Assistant (Laboratory)

Name

Institutes

Ms. Jonaliza Lanceras

RGDU

Ms. Nathinee Pa-In

RGDU

Mr. Meechai Siengliw

RGDU

Ms. Chanakarn Wongsaprom

RGDU

Ms. Jutarat Jantaboon

RGDU


RGDU

Assistant (Field)

Name

Institutes

Mr. Tanee Sriwongchai

RGDU

Ms. Jutarat Jantaboon

RGDU

Mr. Sangchai Silpakorn

RGDU

 


RGDU woud like to acknowledge the following :

Last Updated on Thursday, 24 June 2010 04:10