May 2007 Report 1 PDF Print E-mail
Thursday, 27 May 2010 08:32

Project update report
Date Report Submitted: May 14, 2007



Identification
GCP Project Number: SP3 G4007.03


Project Name:
“Community of Practices” Concept applied to rice production in the Mekong Region: Quick conversion of popular rice varieties with emphasis on drought, salinity and grain quality improvement


Collaborators (name, affiliation):

Dr. Jonaliza L. Siangliw, Rice Gene Discovery Unit (RDGU), Thailand

Dr. Sureeporn Kate Ngam, Ubon Ratchatani University (UBU), Thailand

Dr. Monthathip Chang, National Agricultural and Forestry Research Institute (NAFRI), Laos

Dr. Men Sarom, Cambodian Agricultural Research and Development Institute (CARDI), Cambodia

Dr. Toe Aung, Department of Agricultural Research (DAR), Myanmar


Total budget $65,000 (year 1)


Report

Report October – May 2007

Collaborating institutes were informed that the proposal submitted to GCP was accepted and will start on 1 January 2007. Letters of support were received from four main collaborating institutes such as National Agricultural and Forestry Institute (NAFRI), Cambodian Agricultural Research and Development Institute (CARDI), Department of Agricultural Research (DAR) and Ubon Ratchatani University (UBN) committing their institutes to participate in the programs outlined in the project proposal such as trainings to be conducted inside and outside of their country. This serves as sign of continues collaboration among countries in the Mekong Region.


Planning for the 2 year training program has been initiated. The trainings to be held at Rice Gene Discovery Unit (RGDU), Thailand were scheduled on May 2007, October 2007, April 2008 and September 2008. For this year, the first training in May will begin on the 21st of May to the 30th (Table 1). The second training was set in October so that the participants will be able to attend BioAsia and the 2nd International Conference for Rice for the Future.


Regarding the workshop to be held this May, each institute had nominated two participants. Dr. Khun Leang Hak and Mr. Chou Vichet are joining from CARDI. NAFRI nominated Mr. Souvanh Thadavong and Mr. Khemkham Hongphakdy and for DAR, Daw Tin Tin Myint and Daw Thi Dar will join the training. All nominated participants are rice breeders. Dr. Sureeporn Katengam from UBN will be in charged with the IR57514 population which is also one of the populations developed during the first marker-assisted selection workshop sponsored by Rockefeller Foundation.


In the last concluded workshop on “Long-term practical training on Molecular Breeding on Rice for Mekong Region (LTMBR for MKR)” sponsored by Rockefeller Foundation and BIOTEC, the group of Cambodia identified drought as the main problem in rainfed areas. The Cambodian participants aim to improve cooking quality in drought tolerant variety named CAR3. They used rice variety ‘Phkar Rum Duol’ as donor. Phkar Rum Duol has good eating and cooking qualities but its yield is low under drought condition. Thus, cooking quality traits were transferred to CAR3 by MAS. At the end of the training, The Cambodian group identified 22 BC2F1 containing the cooking quality traits of Phkar Rum Duol based on marker profiles. After the end of training program, the materials were allowed to self-pollinated and phenotypic selection was applied. Dr. Khun Leang Hak and Mr. Chou Vichet would like to continue two more round of MAS and backcrossing. They will bring the leaf tissue of 200 selected BC2F4 plants with CAR 3 background (which is resistant to drought) introgressed with cooking quality traits from Phkar Rum Duol as starting materials for this MAS training.


The Myanmar participants conducted two MAS activities. The first activity aims to improve salinity tolerance in new released variety ‘IR53936-60-3-2-31’ using rice variety ‘Pokkali’ as donor. Since yield in 4% of their planting areas in Myanmar is greatly affected by soil salinity and farmers living in this area are very poor. Therefore improving salinity tolerance is a need. At the end of the LTMBR for MKR program they identified 22 BC3F1 carried QTL for salt tolerance on chromosome 1 from Pokkali. These materials were allowed to self-pollinated and phenotypic selection was applied in BC3F2 and BC3F3. Daw Tin Tin Myint and Daw Thi Dar will bring 200 BC3F4 lines for MAS selection and identify lines with salinity tolerance to be crossed to IR53936 to generate BC4F1. Then BC4F1 will be selected by MAS and the selected BC4F1 lines will be self-pollinated and phenotypic selection and MAS will be applied in BC4F2. The second activity aims to improve grain quality in high yielding variety ‘Manawthukha’ using Basmati as the donor. Manawthukha is popularly grown in most of rice growing areas in Myanmar, because it is well adapted, high yielding and with high milling recovery. However, its grain quality is inferior compared to Basmati, an aromatic fine grain rice. At the end of the LTMBR for MKR program they identified 4 BC3F1. Myint Yi who got the MS scholarship from BIOTEC will continue this activity to be part of her thesis.


The Laos participants had chosen to improve the grain quality of the glutinous rice Thadokkham 1 or TDK1 by transferring genes from their local variety Homnangnouane, also a sticky rice. In the last program, NAFRI ended up with 14 BC2F2 lines homozygous in 2 grain quality traits. Two hundred BC2F3 lines will be used as working materials by Mr. Souvanh Thadavong and Mr. Khemkham Hongphakdy to conduct MAS. The selected BC2F3 containing good cooking quality will be crossed to TDK1 to generate BC3F1. One more round of MAS backcrossing will be followed.

Another population to be continued in this training was developed by transferring cooking quality traits from a KDML105 introgression lines with good eating and seed quality traits, submergence tolerance and bacterial leaf blight resistance to the widely adapted rice variety IR57514. This population was handled by Thai participants


from Ubon Ratchatani University and Rice Research Center at Ubon. At the end of the LTMBR for MKR program, more than 500 BC3F1 seeds were generated and this will be used as the starting materials for MAS in this project.

Workshops to be held outside RGDU were scheduled in June 19-20, 2007 at CARDI, July 3-4, 2007 at NAFRI and August 20-21, 2007 at DAR. The workshop will emphasize on the application of biotechnology in agriculture, plant genome and gene structure, DNA marker technology, genetic maps, QTL mapping and the use of marker-assisted selection in rice breeding. The video on plant molecular breeding and tangible example of MAS will be made also as part of the workshop in CARDI, NAFRI and DAR. RGDU would like to set the same workshop in all three institutes but in the case of DAR, RGDU may not be able to perform wet lab to demonstrate the use of markers in breeding programs, thus video on how to use MAS will be shown. RGDU is now working with CARDI in the preparation of the workshop specifically on invitation letters from CARDI and equipment needed in the workshop.

Table 1. Schedule of the first training to be held at RGDU on May 21-30, 2007.




Tangible outputs delivered


1. Support from collaborating institutes stating their interest and participation in the project either inside or outside of their country.

2. Plan for the 2 year training to be held at RGDU.

3. Planning and scheduling of the first training in May 2007.

4. Names of nominated participants from CARDI, NAFRI and DAR. Identifying the starting materials for this training from the results obtained in the last MAS training.

5. Plan for on site workshop to be held at CARDI, NAFRI and DAR.


Deviations from the work plan

The schedules of trainings at RGDU and workshops at CARDI, NAFRI and DAR were changed due to late processing of the money allocated for the project.

The participants were selected by their own institute as opposed to the plan that participants will be selected after the workshop has been done in each institute. The plan was changed because the workshop on site has not been done yet.


Data Availability


The letters of cooperation, names of nominated participants and plans for trainings in RGDU and outside RGDU are all kept in the RGDU server. The website for this MAS training will be posted as link to http://dna.kps.ku.ac.th by the end of September.

Appendix (4 slides are attached)

Last Updated on Thursday, 27 May 2010 08:57