0049672   

Lecture 2011  

1.              Review on Plant Breeding Methods (Dr. Apichart/Dr. Theerayut)   
2.        Self-pollinated crops
3.        Cross-pollinated crops

1.            Gene, Genome and Molecular Biology  (Dr. Apichart/Dr. Vinitchan/Dr. Watchareewan) 

1.        Genome structure and analysis
2.        Gene and molecular genetics
3.        Gene expression and regulation

1.           Genome Analysis  (Mr. Meechai/Dr. Jona) 

1.        Molecular markers
2.        Fragment analysis
3.        SNP-indel variation
4.        Genetic linkage map
5.        QTL map

MIDTERM (exam and assignment)

•           Development of MAS Project: Low resolution plant breeding  (Dr. Apichart/Dr. Theerayut/Dr. Jona) 

•        MAS theory
•        Gene pyramiding

•            Sequence-based analysis  (Dr. Vinitchan/Mr. Wintai)

•        Bioinformatics
•        New genomic tools
•        Microarray
•        SFP

•           Functional Marker Development 

•        Map-based cloning   (Mr. Wintai)
•        Candidate gene analysis (association)  (Dr. Vinitchan)
•        Similarity analysis and cloning  (Dr. Vinitchan)
•        Mutation analysis  (Dr. Watchareewan/Mr. Siriphat)

•         SNP-based Molecular Analysis  (Dr. Apichart/Mr. Ekawat/Dr. Vinitchan) 

•        SNP-based MAS
•        Phylogenomics
•        Association analysis

•        High-resolution Plant Breeding  (Dr. Apichart/Mr. Wintai/Mr. Siriphat/Mr. Ekawat)  
•        Breeding –by-design
•        Gene pyramiding

•           Transgenic Plant Breeding (Dr. Chanate) 

FINALS (paper presentation, exam and project)

Lecture = 2 hrs. x 18 weeks = 36

Lab      = 3 hrs. x 18 weeks = 54 Total  90

Lecture ( 2007 )

0049672:Molecular plant Breeding 003579:Molecular Biology in Crop Improvement Lecture (click view) First Semester, 2007 14.00  - 15.30 Tuesday - friday Introduction Gene, Genome, and Plant Breeding (Dr. Apichart Vanavichit) Lab: Set up molecular mapping experiments Review basic molecular techniques, Genomic DNA isolation - Genomic DNA isolation          - Genomic DNA isolation         - Gel electrophoresis                 PCR                 Southern blots Molecular Tools: Molecular Marker Technology Molecular markers and DNA fingerprinting     Homework      Mr. Meechai      Mr. Samart      Mrs. Duangjai - RFLP-based marker - PCR-based markers          RAPD          AFLP         STS          EST          SSR          SSCP Data analysis (PIC value, heterozygosity) Primer design Lab: Demo (Staffs) Gene identification known only by phenotype Genome mapping           ( Dr. Theerayut Toojinda) (4.5 hrs)             linkage map             - Genome Mapping (Lecture)             Linkage map construction             - GMendel 3.0             - JoinMap 2.0             - Map maker 3.0 Assignment               Manual calculate (assignment) Lab: map construction and QTL analysis (Mr. Meechai)     QTL         mapmaker help         QTL interaction         qtl slides Marker-assisted selection (Dr. Theerayut Toojinda) Lab: Paper discussion  (Mr. Meechai, Ms. Nathinee, Ms. Jona) ____________________________________Mid-term Exam____________________________________  Molecular diversity analysis  (Apichart) Lab: Scoring markers and analysis  (Mr. Ekawat) Physical mapping Lab: Pulse field gel electrophoresis, STC marker (Mr. Wintai)     Presentation         Introduction         physical map 2003         Marker Enrichment

    content map seq

    Paper PDF         Bulk segregant analysis         chromosome landing         gene golfing Gene Hunting: Positional cloning (Dr.Apichart Vanavichit) Lab: Demo High resolution map and discussion         Genome sequencing, data mining and gene discovery Lab: Demo sequencing Functional genomics (Gene machine) Lab: Demo SNP, Transposon tagging )         Expression genomics and Transcription mapping (Dr. Chatchawan) Lab: Demo: Microarray, cDNA selection, SAGE, Differential display Transgenic Tools : Genetic Engineering (Apichart) Presentation:         Transformation Lab:         Gene transformation methods         Applications         Biosafety issues ____________________________________Final Exam____________________________________   Exam Download     Mid-term Grading system Mid-term exam : 20% Report : 60% Final exam : 20%