0049672   

Lecture 2011  

1.              Review on Plant Breeding Methods
   (Dr. Apichart/Dr. Theerayut) 
    
2.        Self-pollinated crops
3.        Cross-pollinated crops

 

 

 

1.            Gene, Genome and Molecular Biology 
   (Dr. Apichart/Dr. Vinitchan/Dr. Watchareewan) 

 

1.        Genome structure and analysis
2.        Gene and molecular genetics
3.        Gene expression and regulation

 

  

1.           Genome Analysis 
   (Mr. Meechai/Dr. Jona) 
   

 

1.        Molecular markers
2.        Fragment analysis
3.        SNP-indel variation
4.        Genetic linkage map
5.        QTL map

 

 

 

MIDTERM (exam and assignment)

 

 

•           Development of MAS Project: Low resolution plant breeding 
  (Dr. Apichart/Dr. Theerayut/Dr. Jona) 
  

 

•        MAS theory
•        Gene pyramiding

 

 

•            Sequence-based analysis 
  (Dr. Vinitchan/Mr. Wintai)

 

•        Bioinformatics
•        New genomic tools
•        Microarray
•        SFP

 

 

 

•           Functional Marker Development 
  

 

•        Map-based cloning 
   (Mr. Wintai)
•        Candidate gene analysis (association) 
  (Dr. Vinitchan)
•        Similarity analysis and cloning 
  (Dr. Vinitchan)
•        Mutation analysis 
  (Dr. Watchareewan/Mr. Siriphat)

 

 

•         SNP-based Molecular Analysis 
  (Dr. Apichart/Mr. Ekawat/Dr. Vinitchan) 
  

 

•        SNP-based MAS
•        Phylogenomics
•        Association analysis

 

 

 

 

 

 

•        High-resolution Plant Breeding 
  (Dr. Apichart/Mr. Wintai/Mr. Siriphat/Mr. Ekawat)
   
•        Breeding –by-design
•        Gene pyramiding

 

 

 

•           Transgenic Plant Breeding
  (Dr. Chanate) 

 

FINALS (paper presentation, exam and project)

Lecture = 2 hrs. x 18 weeks = 36

Lab      = 3 hrs. x 18 weeks = 54
Total  90

 

 

 

 

 

 

Lecture ( 2007 )

0049672:Molecular plant Breeding
003579:Molecular Biology in Crop Improvement Lecture (click view) First Semester, 2007 14.00  - 15.30 Tuesday - friday Introduction Gene, Genome, and Plant Breeding (Dr. Apichart Vanavichit) Lab: Set up molecular mapping experiments Review basic molecular techniques, Genomic DNA isolation - Genomic DNA isolation          - Genomic DNA isolation         - Gel electrophoresis                 PCR
                Southern blots Molecular Tools: Molecular Marker Technology Molecular markers and DNA fingerprinting     Homework
     Mr. Meechai
     Mr. Samart
     Mrs. Duangjai - RFLP-based marker - PCR-based markers          RAPD          AFLP         STS          EST          SSR          SSCP Data analysis (PIC value, heterozygosity)

Primer design Lab: Demo (Staffs) Gene identification known only by phenotype

Genome mapping 
         ( Dr. Theerayut Toojinda) (4.5 hrs)             linkage map
            - Genome Mapping (Lecture)
            Linkage map construction
            - GMendel 3.0
            - JoinMap 2.0
            - Map maker 3.0 Assignment
              Manual calculate (assignment) Lab: map construction and QTL analysis (Mr. Meechai)     QTL
        mapmaker help
        QTL interaction
        qtl slides Marker-assisted selection
(Dr. Theerayut Toojinda) Lab: Paper discussion 
(Mr. Meechai, Ms. Nathinee, Ms. Jona) ____________________________________Mid-term Exam____________________________________  Molecular diversity analysis 
(Apichart) Lab: Scoring markers and analysis 
(Mr. Ekawat) Physical mapping Lab: Pulse field gel electrophoresis, STC marker (Mr. Wintai)     Presentation
        Introduction
        physical map 2003
        Marker Enrichment

    content map seq

    Paper PDF
        Bulk segregant analysis
        chromosome landing
        gene golfing Gene Hunting: Positional cloning (Dr.Apichart Vanavichit) Lab: Demo High resolution map and discussion         Genome sequencing, data mining and gene discovery Lab: Demo sequencing Functional genomics (Gene machine) Lab: Demo SNP, Transposon tagging )         Expression genomics and Transcription mapping (Dr. Chatchawan) Lab: Demo: Microarray, cDNA selection, SAGE, Differential display Transgenic Tools : Genetic Engineering (Apichart) Presentation:         Transformation Lab:         Gene transformation methods         Applications         Biosafety issues ____________________________________Final Exam____________________________________  
Exam Download     Mid-term Grading system
Mid-term exam : 20%
Report : 60%
Final exam : 20%