Molecular Plant Breeding

0049672

Lecture 2011

Review on Plant Breeding Methods
(Dr. Apichart/Dr. Theerayut)
Self-pollinated crops
Cross-pollinated crops

Gene, Genome and Molecular Biology
(Dr. Apichart/Dr. Vinitchan/Dr. Watchareewan)

Genome structure and analysis
Gene and molecular genetics
Gene expression and regulation

Genome Analysis
(Mr. Meechai/Dr. Jona)

Molecular markers
Fragment analysis
SNP-indel variation
Genetic linkage map
QTL map

MIDTERM (exam and assignment)

Development of MAS Project: Low resolution plant breeding
(Dr. Apichart/Dr. Theerayut/Dr. Jona)

MAS theory
Gene pyramiding

Sequence-based analysis
(Dr. Vinitchan/Mr. Wintai)

Bioinformatics
New genomic tools
Microarray
SFP

Functional Marker Development

Map-based cloning
(Mr. Wintai)
Candidate gene analysis (association)
(Dr. Vinitchan)
Similarity analysis and cloning
(Dr. Vinitchan)
Mutation analysis
(Dr. Watchareewan/Mr. Siriphat)

SNP-based Molecular Analysis
(Dr. Apichart/Mr. Ekawat/Dr. Vinitchan)

SNP-based MAS
Phylogenomics
Association analysis

High-resolution Plant Breeding
(Dr. Apichart/Mr. Wintai/Mr. Siriphat/Mr. Ekawat)
Breeding –by-design
Gene pyramiding

Transgenic Plant Breeding
(Dr. Chanate)

FINALS (paper presentation, exam and project)

Lecture = 2 hrs. x 18 weeks = 36

Lab = 3 hrs. x 18 weeks = 54
Total 90

Lecture ( 2007 )

0049672:Molecular plant Breeding
003579:Molecular Biology in Crop Improvement Lecture (click view) First Semester, 2007 14.00 - 15.30 Tuesday - friday Introduction Gene, Genome, and Plant Breeding (Dr. Apichart Vanavichit) Lab: Set up molecular mapping experiments Review basic molecular techniques, Genomic DNA isolation - Genomic DNA isolation          - Genomic DNA isolation         - Gel electrophoresis                 PCR
                Southern blots Molecular Tools: Molecular Marker Technology Molecular markers and DNA fingerprinting     Homework
   Mr. Meechai
   Mr. Samart
   Mrs. Duangjai - RFLP-based marker - PCR-based markers          RAPD          AFLP         STS          EST          SSR          SSCP Data analysis (PIC value, heterozygosity)

Primer design Lab: Demo (Staffs) Gene identification known only by phenotype

Genome mapping
         ( Dr. Theerayut Toojinda) (4.5 hrs)             linkage map
            - Genome Mapping (Lecture)
            Linkage map construction
            - GMendel 3.0
            - JoinMap 2.0
            - Map maker 3.0 Assignment
              Manual calculate (assignment) Lab: map construction and QTL analysis (Mr. Meechai)     QTL
        mapmaker help
        QTL interaction
        qtl slides Marker-assisted selection
(Dr. Theerayut Toojinda) Lab: Paper discussion
(Mr. Meechai, Ms. Nathinee, Ms. Jona) ____________________________________Mid-term Exam____________________________________ Molecular diversity analysis
(Apichart) Lab: Scoring markers and analysis
(Mr. Ekawat) Physical mapping Lab: Pulse field gel electrophoresis, STC marker (Mr. Wintai)     Presentation
        Introduction
        physical map 2003
        Marker Enrichment

content map seq

    Paper PDF
        Bulk segregant analysis
        chromosome landing
        gene golfing Gene Hunting: Positional cloning (Dr.Apichart Vanavichit) Lab: Demo High resolution map and discussion         Genome sequencing, data mining and gene discovery Lab: Demo sequencing Functional genomics (Gene machine) Lab: Demo SNP, Transposon tagging )         Expression genomics and Transcription mapping (Dr. Chatchawan) Lab: Demo: Microarray, cDNA selection, SAGE, Differential display Transgenic Tools : Genetic Engineering (Apichart) Presentation:         Transformation Lab:         Gene transformation methods         Applications         Biosafety issues ____________________________________Final Exam____________________________________
Exam Download     Mid-term Grading system
Mid-term exam : 20%
Report : 60%
Final exam : 20%